GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.0.5 mg/mL
Recombinant, ExactAb™, Validated, KD, 0.5 mg/mL ExactAb™,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human CLIC4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
Specifications
Product Name
Recombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG
A synthetic peptide derived from human CLIC4 (AA 3-16)
Control positivo
WB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, KD Validation, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 21.9 s
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 35.0 s
Recombinant CLIC4 Antibody (Ab096857) - IF/ICC Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue).
Recombinant CLIC4 Antibody (Ab096857) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant CLIC4 Antibody (Ab096857) - Western Blot All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 28 kDa Exposure time: 0.5 s
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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