Recombinant ERK2 Antibody - Primary antibody, specific to MAPK1, Rabbit IgG

Cat. No.: Ab102233

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human ERK2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Specifications

Product Name

Recombinant ERK2 Antibody - Primary antibody, specific to MAPK1, Rabbit IgG

Sinónimos

ERK 2 antibody | ERK-2 antibody | ERT1 antibody | Extracellular Signal Regulated Kinase 2 antibody | Extracellular signal-regulated kinase 2 antibody | MAP kinase 1 antibody | MAP kinase 2 antibody | MAP kinase isoform p42 antibody | MAPK 1 antibody | MAP

Especificaciones y pureza

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Especies hospedadoras

Rabbit

Especificidad

MAPK1

Inmunógeno

A synthetic peptide derived from human ERK2 (AA 340-360).

Control positivo

WB: K562, C6, NIH/3T3, HeLa, MCF7, A549, Hep G2, HEK293 lysates. IHC: Human Cholangiocarcinoma Tissue. ICC/IF: K562 cells.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant ERK2 Antibody (Ab102233)

Recombinant ERK2 Antibody (Ab102233) - Western Blot
All lanes: Recombinant ERK2 Antibody (Ab102233) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 41 kDa
Observed band size: 39 kDa

ICC/IF: Recombinant ERK2 Antibody (Ab102233)

Recombinant ERK2 Antibody (Ab102233) - ICC/IF
IF analysis of ERK2 (green) in K562 cells using Recombinant ERK2 Antibody (Ab102233) and cells were counterstained with DAPI (blue).

IHC: Recombinant ERK2 Antibody (Ab102233)

Recombinant ERK2 Antibody (Ab102233) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant ERK2 Antibody (Ab102233). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

Objetivos asociados (humanos)

MAPK1 Tchem Mitogen-activated protein kinase 1 (0 Activities)

Discover Target: MAPK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAPK1 Tchem MAP kinase ERK2 (25055 Activities)

Discover Target: MAPK1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción