GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human MAP1LC3A Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Specifications
Product Name
Recombinant MAP1LC3A Antibody - Primary antibody, specific to MAP1LC3A, Rabbit IgG
Sinónimos
ATG8E antibody | Autophagy related protein LC3 A antibody | Autophagy related ubiquitin like modifier LC3 A antibody | Autophagy-related protein LC3 A antibody | Autophagy-related ubiquitin-like modifier LC3 A antibody | LC3 antibody | LC3A antibody | MAP
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Especies hospedadoras
Rabbit
Especificidad
MAP1LC3A
Inmunógeno
A synthetic peptide derived from human MAP1LC3A (AA 101-120).
Control positivo
WB: NIH/3T3, HeLa, C6, Mouse heart, Mouse brain lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.3 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant MAP1LC3A Antibody (Ab114168) - Western Blot All lanes: Recombinant MAP1LC3A Antibody (Ab114168) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa Observed band size: 14, 16 kDa Exposure time: 57.2 s
Recombinant MAP1LC3A Antibody (Ab114168) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MAP1LC3A Antibody (Ab114168). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant MAP1LC3A Antibody (Ab114168) - IF/ICC
IF analysis of Recombinant MAP1LC3A Antibody (Ab114168) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MAP1LC3A Antibody (Ab114168) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
1/50 - 1/100
IF/ICC
1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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