GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human SOX10 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, Flow Cytometry, IP and so on.
Specifications
Product Name
Recombinant SOX10 Antibody - Primary antibody, specific to SOX10, Rabbit IgG
Sinónimos
DOMantibody | DOM antibody | Dominant megacolon mouse human homolog of antibody | MGC15649 antibody | PCWH antibody | SOX 10 antibody | SOX10 antibody | SOX10_HUMAN antibody | SRY (sex determining region Y) box 10antibody | SRY (sex determining region Y)
Especificaciones y pureza
Recombinant, ExactAb™, Validated, High Performance, See COA
Mecanismos bioquímicos y fisiológicos
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
Especies hospedadoras
Rabbit
Especificidad
SOX10
Inmunógeno
A synthetic peptide derived from human SOX10 (AA 400-500).
ExactAb™, High Performance, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant SOX10 Antibody (Ab128734) - Western Blot All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa Observed band size: 57, 70 kDa Exposure time: 1.1 s
Recombinant SOX10 Antibody (Ab128734) - IF/ICC Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant SOX10 Antibody (
Ab128734
) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant SOX10 Antibody (Ab128734) - IP SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution. 2nd Ab: GAR HRP for IP 1/500 Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate Lane3: A375 whole cell lysate, 10 μg (input) Exposure: 120.0 s.
Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/2000
IP
1/50
Flow Cytometry
1/200 - 1/1000
IF/ICC
1/2000 - 1/10000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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