Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b

Cat. No.: Ab186978
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2b
Clone Number
FG02/4F11
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab186978-10μl
≥10
99,90US$
50μl
Ab186978-50μl
2
259,90US$
100μl
Ab186978-100μl
2
419,90US$
1ml
Ab186978-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
2.799,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application
WB: 1/1000 – 1/100,000
Flow: 1/200 - 1/2,000

Specifications

Product Name
Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Sinónimos
DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
Especificaciones y pureza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Especies hospedadoras
Mouse
Especificidad
Smad3
Inmunógeno
Recombinant full length protein corresponding to Human Smad3
Control positivo
WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotipo
Mouse IgG2b
Tipo de cadena ligera
kappa
SDS-PAGE
150.0 KDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Imágenes

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 1.1 s 

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 69.1 s 

Samd3 Mouse mAb (Ab186978) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Aplicación
AplicaciónDilution info
Flow Cytometry

1/200-1/2000

WB

1/1000 – 1/100,000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0607488Certificate of AnalysisApr 07, 2026 Ab186978
ZJ24F0607489Certificate of AnalysisApr 07, 2026 Ab186978
ZJ24F0607490Certificate of AnalysisApr 07, 2026 Ab186978
ZJ24R0600676Certificate of AnalysisJun 14, 2024 Ab186978
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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