Myc tag Antibody - Primary antibody, specific to Myc tagged protein, Rabbit IgG

Cat. No.: Ab116628

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

WB

Host species

Rabbit

Reactive species

Species independent

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Myc tag Antibody, Polyclonal, could be used for WB and so on.  

Specifications

Product Name

Myc tag Antibody - Primary antibody, specific to Myc tagged protein, Rabbit IgG

Synonyms

c-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc

Specifications & Purity

Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Host species

Rabbit

Specificity

Myc tagged protein

Immunogen

KLH-conjugated synthetic peptide EQKLISEEDL.

Positive Control

WB: HEK293T cells transfected with Myc tag.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Antigen affinity chromatography

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Myc tag Antibody (Ab116628)

Myc tag Antibody (Ab116628) - Western Blot
All lanes: Myc tag Antibody (Ab116628) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 120 kDa
Exposure time: 0.3 s

IP: Myc tag Antibody (Ab116628)

Myc tag Antibody (Ab116628) - IP

All lanes: Myc tag Antibody (Ab116628) at 1/1000 dilution 

Lane 1: HEK-293 whole cell lysate 20μg.

Lane 2: HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate. 

Lane 3: Anti-c-Myc Agarose Resin (A743824) IP in HEK-293 whole cell lysate

Lane 4: Anti-c-Myc Agarose Resin (A743824) IP in HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate. Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 115 kDa 

Observed band size: 65, 120 kDa 

Exposure time: 0.3 s 

Application

• Application: Dilution info
• WB:

  1/1000 - 1/2000

Associated Targets(Human)

MYC Tchem Myc proto-oncogene protein (0 Activities)

Discover Target: MYC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

References

1. Qiping Wu, Nanzhou Wang, Shiwen Wang, Zichao Wang, Yujie Li, Yuanhong Xu, Ying Shen.  (2025)  Tumor Microenvironment-Responsive Nanocluster for Time-Resolved Hyperthermic Intraperitoneal Chemo/Thermal-Therapy against Colorectal Peritoneal Metastasis.  ACS Applied Bio Materials,      [PMID:40754759] [10.1021/acsabm.5c00882]