GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human PGK1 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application WB: 0.01-2μg/mL IHC: 5-30μg/mL ICC/IF: 5-20µg/mL Protein Function In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Specifications
Product Name
PGK1 Antibody - Primary antibody, specific to PGK1, Rabbit IgG
Synonyms
Cell migration-inducing gene 10 protein | EC 2.7.2.3 | Primer recognition protein 2 | PRP 2 | Cell migration-inducing gene 10 protein antibody | Epididymis secretory sperm binding protein Li 68p antibody | HEL S 68p antibody | MGC117307 antibody | MGC8947
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Rabbit
Specificity
PGK1
Immunogen
Recombinant PGK1 expressed in E.coli (AA 2-417)
Positive Control
WB: HeLa, Hep G2, HEK293, HT-29, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human cerebrum, colorectal cancer, glioma, liver, kidney.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
PGK1 Antibody (Ab121480) - Western Blot All lanes: PGK1 Antibody (Ab121480) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 42 kDa Exposure time: 10.9 s
PGK1 Antibody (Ab121480) - IF/ICC FITC staining on IF Sample: HeLa cells.
PGK1 Antibody (Ab121480) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL PGK1 Antibody (Ab121480) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGK1 Antibody (Ab121480) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 30 µg/mL PGK1 Antibody (Ab121480) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGK1 Antibody (Ab121480) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30 µg/mL PGK1 Antibody (Ab121480) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGK1 Antibody (Ab121480) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL PGK1 Antibody (Ab121480) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PGK1 Antibody (Ab121480) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 30 µg/mL PGK1 Antibody (Ab121480) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Application
Application
Dilution info
IHC
5-30 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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