SOD2/MnSOD Antibody - Primary antibody, specific to SOD2

Cat. No.: Ab128615

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Conjugation

Unconjugated

Overview

Rabbit anti Human SOD2/MnSOD Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.5-3µg/mL

IHC: 5-20µg/mL

IF/ICC: 5µg/mL

Protein Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Specifications

Product Name

SOD2/MnSOD Antibody - Primary antibody, specific to SOD2

Synonyms

EC 1.15.1.1 | indophenoloxidase B | IPOB | IPO-B | manganese-containing superoxide dismutase | mangano-superoxide dismutase | Mn SOD | Mn superoxide dismutase | MnSOD | MVCD6 | SOD, Mitochodrial | SOD2 | superoxide dismutase [Mn], mitochondrial | superoxi

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Host species

Rabbit

Specificity

SOD2

Immunogen

Recombinant SOD2 expressed in E.coli (AA 25-222).

Positive Control

WB: HeLa, MCF7, HEK293, A431, A549, Hep G2, K562 and NIH/3T3 cell lysates. IHC: Human prostate cancer, breast cancer, kidney, skin cancer, colorectal cancer, liver tissues. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, Validated

SDS-PAGE

150 kDa

Purification Method

Antigen affinity purified + Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - Western Blot
All lanes: SOD2/MnSOD Antibody (Ab128615) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 1.3 s

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IF/ICC 

IF analysis of SOD2/MnSOD Antibody (Ab128615) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with SOD2/MnSOD Antibody (Ab128615) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Application

• Application: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.5-3 µg/mL;

• IF/ICC:

  5 µg/mL;

Associated Targets(Human)

SOD2 Tbio Superoxide dismutase [Mn], mitochondrial (0 Activities)

Discover Target: SOD2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action