VSV-G tag Mouse mAb - Primary antibody, specific to VSV-G, Mouse IgG1

Cat. No.: Ab134509

GRADE & PURITY Validated, ExactAb™, High Performance, 1.0 mg/mL

Application

IF/ICC, IP, WB

Host species

Mouse

Reactive species

Species independent

Isotype

Mouse IgG1

Clone Number

4A10-9A6-6H5

Conjugation

Unconjugated

Overview

Mouse anti VSV-G tag Antibody, Monoclonal (4A10-9A6-6H5), could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000 - 1/10000
IF/ICC: 1/100 - 1/500
IP: 1/20
Relevance
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.

Specifications

Product Name

VSV-G tag Mouse mAb - Primary antibody, specific to VSV-G, Mouse IgG1

Synonyms

Vesicular stomatitis virus glycoprotein tag antibody | VSV epitope tag antibody | VSV tag antibody | VSV-G epitope tag antibody | VSVG antibody | vsvg tag antibody | VSV-G tag antibody | Anti-VSV-G tag antibody

Specifications & Purity

ExactAb™, Validated, High performance, 1.0 mg/mL

Host species

Mouse

Specificity

VSV-G

Immunogen

Synthetic Peptide of VSV-G-Tag.

Positive Control

WB: VSV-G tag protein. ICC/IF: 293T cells transfected with a VSV-G tagged protein. IP: Transfected 293 cell lysate with VSV-G tag fusion protein.

Conjugation

Unconjugated

Grade

ExactAb™, High Performance, Validated

Isotype

Mouse IgG1

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

WB: VSV-G tag Mouse mAb (Ab134509)

VSV-G tag Mouse mAb (Ab134509) - Western Blot
Western blot analysis of VSV G tag using VSV-G tag Mouse mAb (Ab134509).

IHC: VSV-G tag Mouse mAb (Ab134509)

VSV-G tag Mouse mAb (Ab134509) - IHC
Immunofluorescence analysis of VSV-G tag (4A10) in 293T cells transfected with a VSV-G tagged protein using VSV-G tag Mouse mAb (Ab134509) (red), and cells were counterstained with DAPI (blue).

IP: VSV-G tag Mouse mAb (Ab134509)

VSV-G tag Mouse mAb (Ab134509) - IP
IP antibody use: 5 ug VSV-G mouse IgG1 per mL Lysate.
1. untransfected 293 cell lysate
2. transfected 293 cell lysate with VSV-G-tag fusion protein
3. IP (untransfted 293 + anti-VSV-G mAb + Protein G agarose)
4. IP (transfected 293 + normal mouse IgG + Protein G agarose)
5. IP (transfected 293 + anti-VSV-G mAb + Protein G agarose).

Application

• Application: Dilution info
• IP:

  1/20;

• WB:

  1/1000 - 1/10000 ;

• IF/ICC:

  1/100-1/500 ;