Recombinant CPS1 Antibody - Primary antibody, specific to CPS1, Rabbit IgG

Cat. No.: Ab097550

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.3 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human CPS1 Antibody, Recombinant, could be used for WB, IHC and so on.

Application

• WB: 1/500-1/1000
• IHC: 1/50-1/100

Protein Function

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

Specifications

Product Name

Recombinant CPS1 Antibody - Primary antibody, specific to CPS1, Rabbit IgG

Synonyms

Carbamoyl phosphate synthase [ammonia] antibody | Carbamoyl phosphate synthase [ammonia] mitochondrial antibody | Carbamoyl phosphate synthase antibody | Carbamoyl phosphate synthetase 1 antibody | Carbamoyl phosphate synthetase 1 mitochondrial antibody |

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Host species

Rabbit

Specificity

CPS1

Immunogen

A synthetic peptide derived from human CPS1 (AA 135-149)

Positive Control

WB: HeLa and Hep G2 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Antigen affinity purified

Concentration

0.3 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant CPS1 Antibody (Ab097550)

Recombinant CPS1 Antibody (Ab097550) - Western Blot
All lanes: Recombinant CPS1 Antibody (Ab097550) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 165 kDa
Observed band size: 156 kDa
Exposure time: 10.0 s

IHC: Recombinant CPS1 Antibody (Ab097550)

Recombinant CPS1 Antibody (Ab097550) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant CPS1 Antibody (Ab097550). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant CPS1 Antibody (Ab097550)

Recombinant CPS1 Antibody (Ab097550) - IF/ICC

IF analysis of Recombinant CPS1 Antibody (Ab097550) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant CPS1 Antibody (Ab097550) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

CPS1 Tclin Carbamoyl-phosphate synthase [ammonia], mitochondrial (0 Activities)

Discover Target: CPS1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action