ANGPT2 Antibody - Primary antibody, specific to ANGPT2, Rabbit IgG

Cat. No.: Ab088801

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human ANGPT2 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.

Application

WB: 0.2-2µg/mL

IHC: 5-20µg/mL

IF/ICC: 5µg/mL

Protein Function

Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Specifications

Product Name

ANGPT2 Antibody - Primary antibody, specific to ANGPT2, Rabbit IgG

Synonyms

AGPT 2 antibody | Agpt2 antibody | ANG 2 antibody | ANG-2 antibody | ANG2 antibody | Angiopoietin 2a antibody | Angiopoietin 2B antibody | Angiopoietin-2 antibody | Angiopoietin2 antibody | ANGP2_HUMAN antibody | ANGPT 2 antibody | Angpt2 antibody | Tie2

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Host species

Rabbit

Specificity

ANGPT2

Immunogen

Recombinant ANGPT2 expressed in E.coli (AA 24-165)

Positive Control

WB: K562 and HUVEC cell lysates. IHC: Human Placenta Tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ANGPT2 Antibody (Ab088801)

ANGPT2 Antibody (Ab088801) - Western Blot
All lanes: ANGPT2 Antibody (Ab088801) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 79 kDa
Observed band size: 73 kDa
Exposure time: 0.8 s

IHC: ANGPT2 Antibody (Ab088801)

ANGPT2 Antibody (Ab088801) - IHC
DAB staining on IHC
Samples: Human Placenta Tissue
Primary Ab: 20 μg/mL ANGPT2 Antibody (Ab088801)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: ANGPT2 Antibody (Ab088801)

ANGPT2 Antibody (Ab088801) - IF/ICC 

IF analysis of ANGPT2 Antibody (Ab088801) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ANGPT2 Antibody (Ab088801) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Application

• Application: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.2-2 µg/mL ;

• IF/ICC:

  5 µg/mL;

Associated Targets(Human)

ANGPT2 Tclin Angiopoietin-2 (0 Activities)

Discover Target: ANGPT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action