GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ATP6V1B1 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/1000 IHC: 1/100-1/500 ICC/IF: 1/10-1/50 Protein Function Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Specifications
Product Name
ATP6V1B1 Antibody - Primary antibody, specific to ATP6V1B1, Rabbit IgG
A KLH conjugated synthetic peptide from the Central region of human ATP6V1B1 (AA 284-310)
Positive Control
WB: HeLa, K562, HEK293, Hep G2, MCF7 and NIH/3T3 cell lysates. IHC: Human kidney tissue. ICC/IF: WiDr cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ATP6V1B1 Antibody (Ab090171) - Western Blot All lanes: ATP6V1B1 Antibody (Ab090171) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 51.1 s
ATP6V1B1 Antibody (Ab090171) - Western Blot All lanes: ATP6V1B1 Antibody (Ab090171) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 57 kDa Observed band size: 49 kDa Exposure time: 20.0 s
ATP6V1B1 Antibody (Ab090171) - IF/ICC Confocal immunofluorescent analysis of ATP6V1B1 Antibody (Ab090171) with WiDr cell followed by AF488-conjugated goat anti-rabbit lgG (green), DAPI was used to stain the cell nuclear (blue).
ATP6V1B1 Antibody (Ab090171) - IHC ATP6V1B1 Antibody (Ab090171) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ATP6V1B1 Antibody (Ab090171) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Application
Dilution info
ICC/IF
1/10-1/50
IHC
1/100-1/500
WB
1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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