Goat Anti-Human IgG (AF405) - Secondary antibody, IgG

Cat. No.: Ab181959

GRADE & PURITY Validated, ExactAb™, Ex:401nm, Em:422nm, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

AF405

Overview

Application
Flow: 1:200-1:2000
IF: 1:100-1:1000.

Specifications

Product Name

Goat Anti-Human IgG (AF405) - Secondary antibody, IgG

Synonyms

Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody |

Specifications & Purity

ExactAb™, Validated, Ex:401nm, Em:422nm, 1.0 mg/mL

Host species

Goat

Conjugation

AF405

Excitation (nm)

Violet Laser(405nm)

Emission(nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grade

Em:422nm, Ex:401nm, ExactAb™, Validated

Isotype

IgG

Purification Method

antigen affinity chromatography

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - IF
IF analysis of EGFR (blue) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Serclutamab (anti-EGFR) (Ab182867) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD276 (red) with Mirzotamab (anti-CD276) (Ab182952). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Goat Anti-Human IgG (AF405) (Ab181959)

Goat Anti-Human IgG (AF405) (Ab181959) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling IGF1R (red) with Ganitumab (anti-IGF1R) (Ab177931). Goat Anti-Human IgG (AF405) (Ab181959) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  1/200-1/2000;

• IF/ICC:

  1/100-1/1000 ;