RPL13A Antibody - Primary antibody, specific to RPL13A, Rabbit IgG

Cat. No.: Ab125785

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.7 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human RPL13A Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/2000

IHC: 1/50-1/100

IF/ICC: 1/500

Protein Relevance:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. 

Specifications

Product Name

RPL13A Antibody - Primary antibody, specific to RPL13A, Rabbit IgG

Synonyms

23 kDa highly basic protein | Large ribosomal subunit protein uL13 | 23 kD highly basic protein antibody | 23 kDa highly basic protein antibody | 60S ribosomal protein L13a antibody | L13A antibody | P198 antibody | Ribosomal protein L13a antibody | RPL 1

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.7 mg/mL

Host species

Rabbit

Specificity

RPL13A

Immunogen

Fusion protein of human RPL13A (AA 4-203)

Positive Control

WB: A549, HT-29 and K562 cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.7 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: RPL13A Antibody (Ab125785)

RPL13A Antibody (Ab125785) - Western Blot
All lanes: RPL13A Antibody (Ab125785) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 27 kDa
Exposure time: 5.5 s

IHC: RPL13A Antibody (Ab125785)

RPL13A Antibody (Ab125785) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RPL13A Antibody (Ab125785). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: RPL13A Antibody (Ab125785)

RPL13A Antibody (Ab125785) - IF/ICC 

IF analysis of RPL13A Antibody (Ab125785) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RPL13A Antibody (Ab125785) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1:500 ;

Associated Targets(Human)

RPL13A Tbio 60S ribosomal protein L13a (0 Activities)

Discover Target: RPL13A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)