CD8 alpha Rat mAb (AF488) - Primärer Antikörper, specific to CD8 alpha

Cat. No.: Ab190216

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

AF488

Übersicht

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Specifications

Product Name

CD8 alpha Rat mAb (AF488) - Primärer Antikörper, specific to CD8 alpha

Synonyme

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Wirtsspezies

Rat

Spezifität

CD8 alpha

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes

Konjugation

AF488

Anregung (nm)

Blue Laser(488nm)

Emission (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

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CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

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