CD8 alpha Rat mAb (PE) - Primärer Antikörper, specific to CD8 alpha, Rat IgG2b

Cat. No.: Ab181177

QUALITÄT & REINHEIT ExactAb™, Azide Free, Validated, Ex:565nm, Em:575nm, 5μL/test

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2b

Clone Number

2.43

Conjugation

PE

Übersicht

Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.

Spezifikationen

Product Name

CD8 alpha Rat mAb (PE) - Primärer Antikörper, specific to CD8 alpha, Rat IgG2b

Synonyme

CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell surface glycoprotein CD8 alph

Spezifikationen & Reinheit

ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 5μL/test

Wirtsspezies

Rat

Spezifität

CD8 alpha

Immunogen

Mouse CTL clone L3

Positive Kontrolle

Flow: Murine splenocytes

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

Azide Free, Em:575nm, Ex:565nm, ExactAb™, Validated

Isotyp

Rat IgG2b

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

5μL/test

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab181177)

CD8 alpha Rat mAb (PE) (Ab181177) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (PE) (Ab181177) at 5μL/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (PE) (Ab180312).

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab181177)

CD8 alpha Rat mAb (PE) (Ab181177) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (PE) (Ab181177) at 5μL/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Zugehörige Ziele (menschlich)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD8 alpha Rat mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.