CD8 alpha Rat mAb (PE) - Primärer Antikörper, specific to CD8 alpha

Cat. No.: Ab190209

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:575nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

PE

Übersicht

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Specifications

Product Name

CD8 alpha Rat mAb (PE) - Primärer Antikörper, specific to CD8 alpha

Synonyme

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | PE Anti-Mouse CD8a (53-6.7) | PE Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), PE | PE anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibody | T8 T cell a

Spezifikationen & Reinheit

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Wirtsspezies

Rat

Spezifität

CD8 alpha

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Kontrolle

Flow: C57BL/6 mouse splenocytes.

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

Em:575nm, Ex:565nm, ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab190209)

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab190209)

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

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