CD8 alpha Rat mAb (Biotin) - Primärer Antikörper, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab190207

QUALITÄT & REINHEIT ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

Biotin

Übersicht

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Spezifikationen

Product Name

CD8 alpha Rat mAb (Biotin) - Primärer Antikörper, specific to CD8 alpha, Rat IgG2a

Synonyme

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | Biotin Anti-Mouse CD8a (53-6.7) | Biotin Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), Biotin | Biotin anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibo

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains

Wirtsspezies

Rat

Spezifität

CD8 alpha

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Kontrolle

Flow: C57BL/6 mouse splenocytes.

Konjugation

Biotin

Note

ExactAb™, Validated

Isotyp

Rat IgG2a

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab190207)

CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab190207)

CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

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CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

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