Recombinant CD8 alpha Antibody (PE) - Primärer Antikörper, specific to Mouse CD8α

Cat. No.: Ab213872

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

P83E51

Conjugation

PE

Übersicht

Recombinant CD8α Antibody, clone P83E51, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD8 alpha Antibody (PE) - Primärer Antikörper, specific to Mouse CD8α

Synonyme

alpha polypeptide (p32) antibody | CD_antigen=CD8a antibody | CD8 antibody | CD8 antigen alpha polypeptide antibody | CD8 antigen alpha polypeptide (p32) antibody | CD8 antigen, alpha polypeptide (p32) antibody | CD8a antibody | CD8A antigen antibody | CD

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com

Wirtsspezies

Rat

Spezifität

Mouse CD8α

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: Recombinant CD8 alpha Antibody (PE) (Ab213872)

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: Recombinant CD8 alpha Antibody (PE) (Ab213872)

Recombinant CD8 alpha Antibody (PE) (Ab213872) - Flow Cytometry
Flow cytometry analysis of CD8 alpha by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD8 alpha Antibody (PE) (Ab213872) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

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CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

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