CD8 alpha Rat mAb (AF647) - Primary antibody, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab190218
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:650nm ? Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:668nm ? Fluorescence emission maximum at 668 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
53-6.7
Conjugation
AF647
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab190218-10μg
4

$37.90

$47.90
Save $10.00 (20.88%)
25μg
Ab190218-25μg
2

$71.90

$95.90
Save $24.00 (25.03%)
100μg
Ab190218-100μg
1

$171.90

$206.90
Save $35.00 (16.92%)
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL Em:668nm,Ex:650nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Specifications

Product Name
CD8 alpha Rat mAb (AF647) - Primary antibody, specific to CD8 alpha, Rat IgG2a
Synonyms
Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | AF647 Anti-Mouse CD8a (53-6.7) | AF647 Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), AF647 | AF647 anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibody |
Specifications & Purity
ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Host species
Rat
Specificity
CD8 alpha
Immunogen
Mouse Spleen Cells or Thymocyte Membranes
Positive Control
Flow: C57BL/6 mouse splenocytes.
Conjugation
AF647
Excitation (nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grade
Em:668nm, Ex:650nm, ExactAb™, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).

CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F1012314Certificate of AnalysisMay 20, 2026 Ab190218
ZJ24F1012315Certificate of AnalysisMay 20, 2026 Ab190218
ZJ24F1012316Certificate of AnalysisMay 20, 2026 Ab190218
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:650nm grade guide → View Em:668nm grade guide →

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