ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human ATP1A1 Antibody, Recombinant antibody (RR685), could be used for WB, IHC, Flow, IP and so on.
Application:
WB: 1/2000 - 1/5000
IHC: 1/10000 - 1/20000
Flow: 1/50 - 1/200
IP: 1/50
Protein Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Post-translational
Phosphorylation on Tyr-10 modulates pumping activity.
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s
Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)
Exposure: 120 s.
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| WB |
1/2000 - 1/5000
|
| Flow Cytometry | 1/50 - 1/200 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901094 | Certificate of Analysis | Jun 24, 2025 | Ab128631 |
| ZJ23F0901095 | Certificate of Analysis | Jun 24, 2025 | Ab128631 |
| ZJ26F0636091 | Certificate of Analysis | Jun 24, 2025 | Ab128631 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
