GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
ExactAb™, Validated, KD Validation, Recombinant, High Performance, See COA ExactAb™,High Performance,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NF-kB p65 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, IP and so on.
Specifications
Product Name
Recombinant NF-kB p65 Antibody - Primary antibody, specific to NF-kB p65
ExactAb™, Validated, KD Validation, Recombinant, High Performance, See COA
Biochemical and Physiological Mechanisms
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or het
Host species
Rabbit
Specificity
NF-kB p65
Immunogen
A synthetic peptide derived from human NF-kB p65 (AA 460-551).
Positive Control
WB: HT-1080, K562, HEK293, Jurkat, HeLa and NIH/3T3 cell lysates. IHC: Human bladder cancer tissue. IF/ICC: HeLa cells. IP: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, KD Validation, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NF-kB p65 Antibody (Ab117947) - Western Blot All lanes: Recombinant NF-kB p65 Antibody (Ab117947) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa Observed band size: 68 kDa Exposure time: 13.7 s
Recombinant NF-kB p65 Antibody (Ab117947) - Western Blot All lanes use Recombinant NF-kB p65 Antibody (Ab117947) at 1/5000 dilution for 1 hour at room temperature.
Recombinant NF-kB p65 Antibody (Ab117947) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant NF-kB p65 Antibody (Ab117947) at 1/50 dilution.
Recombinant NF-kB p65 Antibody (Ab117947) - IHC Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using Recombinant NF-kB p65 Antibody (Ab117947).
Recombinant NF-kB p65 Antibody (Ab117947) - IP Immunoprecipitate (IP) analysis using Recombinant NF-kB p65 Antibody (Ab117947) at 1/50 dilution. (WB with Recombinant NF-kB p65 Antibody (Ab117947) at 1/6000 dilution)
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50-1/200
WB
1/1000 - 1/5000
IP
1/20-1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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