CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab190207

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

Biotin

Overview

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Specifications

Product Name

CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2a

Synonyms

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | Biotin Anti-Mouse CD8a (53-6.7) | Biotin Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), Biotin | Biotin anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibo

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains

Host species

Rat

Specificity

CD8 alpha

Immunogen

Mouse Spleen Cells or Thymocyte Membranes

Positive Control

Flow: C57BL/6 mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab190207)

CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD8 alpha Rat mAb (Biotin) (Ab190207)

CD8 alpha Rat mAb (Biotin) (Ab190207) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab190207) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)