Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess?

Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess?

After RIPA lysate lysed the cells, the DNA in the nucleus was more complete diffused into the sample, forming a sticky liquid, in this case after lysis can then be sonicated, sonicate the sample for 1 min at 35~40% power (carried out in an ice bath), sonicate for 2 s, with an interval of 2 s, to ensure that the cells are fully lysed and to reduce the viscosity of the sample.