GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit Anti-Chicken IgY H&L Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA and so on. WB: 1/2000 - 1/10000 CLIA: 0.5µg/mL-10µg/mL LF/ GICA: 0.5mg/mL-2.0mg/mL EIA: 1/1000 - 1/50000
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, See COA
Host species
Rabbit
Immunogen
Chicken IgY
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
Rabbit IgG
SDS-PAGE
25.7 kDa (Light Chain) & 51.3 kDa (Heavy Chain), under reducing conditions; 127.4 kDa, under non-reducing conditions.
Purification Method
Antigen affinity chromatography
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) - ELISA Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) was tested by ELISA. The plate was coated with 0.5 μg/mL Chicken IgY (Ab008417). Rabbit Anti-Chicken IgY H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) - ELISA Cross-reactivity of Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit Anti-Chicken IgY H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
Application
Application
Dilution info
CLIA
0.5-10 µg/mL
LF/GICA
0.5-2.0 mg/mL
EIA
1/1000 - 1/50000
WB
1/2000 - 1/10000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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