ExactAb™, Recombinant, Validated, Azide Free, Ex:565nm, Em:575nm, 0.05mg/mL; 10uL/Test Azide Free,Em:575nm,Ex:565nm,ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Background:
The recombinant monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation and proliferation.
Conjugation:
Purified antibody is conjugated with PE under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Recommended Usage：
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 10 µ of antibody per test (million cells in 100 µL staining volume ). Since applications vary, the appropriate dilutions must be determined for individual use.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Recombinant CD20 Antibody (PE) (Ab169186)-Flow
Raji Cells are stained with Recombinant CD20 Antibody (PE) (Ab169186) (Red). Unstained Raji Cells (Gray) are used as control.
Recombinant CD20 Antibody (PE) (Ab169186) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an unlabelled cells (left panel). Cells were stained with Recombinant CD20 Antibody (PE) (Ab169186) at 10μL/test for 1 hour at 4°C. Left panel - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD20 Antibody (PE) (Ab169186) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an unlabelled cells (black). Cells were stained with Recombinant CD20 Antibody (PE) (Ab169186) at 10μL/test for 1 hour at 4°C. Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow |
10µL/Test
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700600 | Certificate of Analysis | Apr 09, 2026 | Ab169186 |
| ZJ23F0700601 | Certificate of Analysis | Apr 09, 2026 | Ab169186 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:565nm grade guide → View Em:575nm grade guide →
