TOMM40 Antibody - Primary antibody, specific to TOMM40, Rabbit IgG

Cat. No.: Ab131963

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human TOMM40 Antibody, Polyclonal, could be used for WB, ICC, IF, Flow and so on.
Application:
WB: 1/2000
Flow: 1/25
ICC/IF: 1/25
Protein Function
Channel-forming protein essential for import of protein precursors into mitochondria.

Specifications

Product Name

TOMM40 Antibody - Primary antibody, specific to TOMM40, Rabbit IgG

Synonyms

p38.5 | Protein Haymaker | Translocase of outer membrane 40 kDa subunit homolog | C19orf1 antibody | D19S1177E antibody | Haymaker protein antibody | Mitochondrial import receptor subunit TOM40 homolog antibody | Mitochondrial outer membrane protein antib

Specifications & Purity

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Host species

Rabbit

Specificity

TOMM40

Immunogen

A KLH conjugated synthetic peptide from human TOMM40 (AA 22-56)

Positive Control

WB: HEK293, HeLa, A431, Hep G2 and SK-OV-3 cell lysates. ICC/IF: U2OS cells. Flow: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified + Peptide affinity purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - Western Blot
All lanes: TOMM40 Antibody (Ab131963) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38, 39 kDa
Exposure time: 12.9 s

IF/ICC: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U2OS (human osteosarcoma cell line) cells labeling TOMM40 with TOMM40 Antibody (Ab131963) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Flow Cytometry: TOMM40 Antibody (Ab131963)

TOMM40 Antibody (Ab131963) - Flow Cytometry
Overlay histogram showing HeLa cells stained with TOMM40 Antibody (Ab131963) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TOMM40 Antibody (Ab131963) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Application

• Application: Dilution info
• Flow Cytometry:

  1/25;

• WB:

  1/2000;

• IF/ICC:

  1:25;

Associated Targets(Human)

TOMM40 Tbio Mitochondrial import receptor subunit TOM40 homolog (0 Activities)

Discover Target: TOMM40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action