ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human EpCAM Antibody, Recombinant antibody (RR694), could be used for WB, IHC, Flow and IP.
Application:
WB: 1/2000 – 1/5000
IHC: 1/1600-1/3200
Flow: 1/800-1/2000
IP: 1/50
IF/ICC: 1/1000 - 1/2000
Protein Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s
Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 120 s
Recombinant EpCAM Antibody (Ab101839) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (LumiDye™ Blue 515) (Ab220504) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| Flow Cytometry | 1/800 - 1/2000 |
| WB |
1/2000 - 1/5000
|
| IF/ICC | 1/1000 - 1/2000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901018 | Certificate of Analysis | Sep 11, 2023 | Ab101839 |
| ZJ23F0901017 | Certificate of Analysis | Sep 11, 2023 | Ab101839 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
