Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody

Cat. No.: Ab190480

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

AF750

Overview

Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be uesd for Flow Cytometry and IF/ICC.

Specifications

Product Name

Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody

Synonyms

Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Goat

Immunogen

Mouse IgG

Conjugation

AF750

Excitation (nm)

Red Laser(633nm)

Emission(nm)

776nm

Ex/Em(nm)

Ex:752nm;Em:776nm

Grade

ExactAb™, Validated

Purification Method

Antigen affinity chromatography

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF: Goat Anti-Mouse IgG H&L (AF750) (Ab190480)

Goat Anti-Mouse IgG H&L (AF750) (Ab190480) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF750) (Ab190480) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (AF750) (Ab190480)

Goat Anti-Mouse IgG H&L (AF750) (Ab190480) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF750) (Ab190480) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  1/100-1/1000 ;

• IF/ICC:

  1/100-1/1000 ;

References

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]