Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody

Cat. No.: Ab156255
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. Pre-adsorbed ? Pre-adsorbed — cross-reactive antibodies removed by adsorption for higher specificity. Use in multiplex/IHC to cut background and cross-reaction. 1.0 mg/mL
Application
ELISA, WB
Host species
Goat
Reactive species
Mouse
Conjugation
HRP
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab156255-100μg
5
$39.90
1mg
Ab156255-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$199.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Pre-adsorbed,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
WB: 1:10,000-1:200,000
ELISA: 1:5,000-1:100,000

Specifications

Product Name
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody
Synonyms
Goat Anti-Mouse IgG H&L Antibody (HRP) | Anti-Mouse IgG H&L Antibody (HRP) | Mouse IgG H&L Antibody (HRP) | HRP-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (HRP) | Anti-Mouse IgG Antibody (HRP) | Mouse IgG Antibody (HRP) | Goat ant
Specifications & Purity
ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL
Host species
Goat
Immunogen
Mouse IgG
Positive Control
ELISA: Mouse IgG
Conjugation
HRP
Grade
Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Note
This product can replace Ab138040
Storage and Shipping
Shape
Liquid
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Images

Goat Anti-Mouse IgG H&L (HRP) (Ab156255) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab156255) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 2.6 s

Goat Anti-Mouse IgG H&L (HRP) (Ab156255) - ELISA
Goat Anti-Mouse IgG H&L (HRP) (Ab156255) was tested by direct ELISA. The plate was coated with 50 ng of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (HRP) (Ab156255) was used as the secondary antibody.

Application
ApplicationDilution info
WB

1/10000-1/200000

ELISA

1/5000-1/100000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001204Certificate of AnalysisFeb 10, 2026 Ab156255
ZJ23F1001205Certificate of AnalysisJul 30, 2024 Ab156255
Documents & Articles
In-cell ELISA protocol
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
Automated Immunohistochemical Staining Protocol for Anti-PD-L1
General Protocol for Western Blotting
BN-PAGE Protocol
Histone Western Blot
Protocol of Western blot for Phosphorylated Proteins
Guidelines of Secondary Antibody for Western Blotting
Multiplex Immunohistochemistry Protocol
Horseradish Peroxidase (HRP) — Structural Features, Enzymatic Properties and Application Overview
Tyramide Signal Amplification in In Situ Fluorescence Detection
Protein Carbonyl Detection
Practical Guide to TSA Tyramide Signal Amplification: How to Select Reagents, Optimize the Workflow, and Reduce Background When Detection Signals Are Weak for Low-Abundance Targets
Citations of This Product
References
1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View Pre-adsorbed grade guide →

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