Rabbit Anti-Mouse IgG antibody - Secondary antibody

Cat. No.: Ab170451

GRADE & PURITY High Performance, PBS Only, See COA

Application

CLIA, EIA, IHC, LF, WB

Host species

Rabbit

Reactive species

Mouse

Conjugation

Unconjugated

Overview

Purified rabbit polyclonal antibody, suitable for LF, CLIA, EIA, WB, IHC and other immune detection platforms and other suitable application scenarios. Antigen affinity chromatography, purity greater than 95%, buffer system 0.01M PBS (pH7.4), 0.2μm filtration, no preservatives added.

Specifications

Product Name

Rabbit Anti-Mouse IgG antibody - Secondary antibody

Specifications & Purity

High Performance, PBS Only, See COA

Host species

Rabbit

Conjugation

Unconjugated

Grade

High Performance, PBS Only

Purification Method

antigen affinity chromatography

Concentration

See COA

Storage and Shipping

Shape

liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Images

ELISA: Rabbit anti-mouse IgG antibody (Ab170451)

Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The plate was coated with different amounts of mouse IgG (Ab170221) (3-fold started from 100 ng/mL). Rabbit anti-mouse IgG antibody was then added at a series of different dilution ratios. Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.

ELISA: Rabbit anti-mouse IgG antibody (Ab170451)

Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Cross-reactivity of Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit anti-mouse IgG antibody was then added at different dilution ratio followed by incubation for 1h. Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
For the batch tested, Rabbit anti-mouse IgG antibody (Ab170451) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.