Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG

Cat. No.: Ab179008

GRADE & PURITY Validated, See COA

Application

ELISA, WB

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

Alkaline Phosphatase

Overview

WB: 1:5000-1:30000
ELISA: 1:1000-1:10000

Specifications

Product Name

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG

Synonyms

Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse Ig

Specifications & Purity

Validated, See COA

Host species

Goat

Immunogen

Mouse IgG

Conjugation

Alkaline Phosphatase

Grade

Validated

Isotype

Goat IgG

Purification Method

Antigen affinity chromatography

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Western Blot: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) at 1/20000 dilution
Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.

Predicted band size: 42 kDa
Observed band size: 42 kDa

ELISA: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)- ELISA
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.

ELISA: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - ELISA
Cross-reactivity of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.

Application

• Application: Dilution info
• ELISA:

  1/1000-1/10000 ;

• WB:

  1:5000-1:30000 ;

References

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]