Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody

Cat. No.: Ab156254

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:498nm, Em:517nm, Pre-adsorbed, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Mouse

Conjugation

FITC

Overview

Application
IF: 1:1000
Flow: 1:200-1:2000

Specifications

Product Name

Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody

Synonyms

Goat Anti-Mouse IgG H&L Antibody (FITC) | Anti-Mouse IgG H&L Antibody (FITC) | Mouse IgG H&L Antibody (FITC) | FITC-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (FITC) | Anti-Mouse IgG Antibody (FITC) | Mouse IgG Antibody (FITC) | G

Specifications & Purity

ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, Pre-adsorbed, 1.0 mg/mL

Host species

Goat

Immunogen

Mouse IgG

Conjugation

FITC

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grade

Azide Free, Em:517nm, Ex:498nm, ExactAb™, Pre-adsorbed, Validated

Purification Method

Antigen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

IF: Goat Anti-Mouse IgG H&L (FITC) (Ab156254)

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (FITC) (Ab156254)

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

• Application: Dilution info
• IF/ICC:

  1:1000;

• Flow Cytometry:

  1/200-1/2000;

References

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]