GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human PKM Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 0.01-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20 μg/mL Protein Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Specifications
Product Name
PKM Antibody - Primary antibody, specific to PKM, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Rabbit
Specificity
PKM
Immunogen
Recombinant PKM2 expressed in E.coli (AA 141-248)
Positive Control
WB: K562, MCF7, HeLa, HepG2, A431, THP-1, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue; Human skin cancer tissue, Human prostate tissue, Human prostate cancer tissue, Human colorectal cancer tissue, Human liver cancer tissue. ICC/IF
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified and Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
PKM Antibody (Ab122143) - Western Blot All lanes: PKM Antibody (Ab122143) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 1.6 s
PKM Antibody (Ab122143) - IF/ICC FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 ug/mL PKM Antibody (Ab122143) Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Skin cancer Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Prostate cancer Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL PKM Antibody (Ab122143) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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