GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, KD Validation, See COA
Biochemical and Physiological Mechanisms
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ra
Host species
Rabbit
Specificity
PKM
Immunogen
Recombinant PKM2 expressed in E.coli (AA 141-248).
Positive Control
WB: K562, MCF7, HeLa, Hep G2, Raji, Ramos, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, skin cancer, prostate, prostate cancer, colorectal cancer, liver cancer, Mouse lung, testis, kidney, oviduct tissues. IF/ICC: MCF7, HeLa cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, KD Validation, Validated
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
PKM2 Antibody (Ab122149) - Western Blot All lanes: PKM2 Antibody (Ab122149) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 61 kDa Exposure time: 3.7 s
PKM2 Antibody (Ab122149) - IF/ICC FITC staining on IF Samples: Human MCF7 cell Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149) Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Skin cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Prostate cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - Western Blot Lane 1: Wild-type MCF7 cell lysateLane 2: PKM2 knockout MCF7 cell lysatePredicted MW: 56,58 kDaObserved MW: 60 kDaPrimary Ab: 2 µg/mL PKM2 Antibody (Ab122149) Second Ab: 0.2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IF/ICC AF488 staining on IF Samples: HeLa cell Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Lung Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Testis Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Oviduct Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Oviduct Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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