1,4-Dithiothreitol (DTT) - ≥97% , CAS No.3483-12-3

CAS: 3483-12-3 Cat. No.: D265376 Fórmula: C₄H₁₀O₂S₂ Peso molecular: 154.25 Beilstein Registry Number: 1719760 Número EC: 222-468-7

CALIDAD & PUREZA ≥97%

Synonyms

Threitol, 1,4-dithio-, L-|(+/-)-1,4-Dimercapto-2,3-butanediol|2-propylpenten-2-oic Acid|dithio threitol|2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel- (9CI)|(+/-)-Dithiothreitol|2,3-BUTANEDIOL, 1,4-DIMERCAPTO-, L-threo-|DTT, 1,4-Dithiothreitol|3,7-Dimethyl-6,7-epoxy-1-(4-ethylphenoxy)-2-octene|WR 34678|(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL|1,4-Dithiothreithol|(R-(R*,R*))-1,4-Dimercaptobutane-2,3-diol|DL-1,4-Dithiothreitol|dl-Dithiothreitol|DL-Dithiothreitol; Cleland inverted exclamation mark s reagent|ETHYL4-NITRO-L-PHENYLALANINE|1,4-Dithio-dl-threitol|cis-2,3-Dihydroxy-1,4-butanedithiol|threo-1,4-Dimercapto-2,3-butanediol|DL-Dithiothreitol, powder|Reagents, Cleland's|DTT|DTT, Dithiothreitol (Cleland's Reagenz)|Sputolysin|(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol|1H-2-Benzoxacyclotetradecin-1-one, 3,4,5,6,7,8,9,10-octahydro-7,14,16-trihydroxy-3-methyl-, (3S,7S)-|D,L-Dithiothreitol (DTT)|(E)-2-(3,7-Dimethylocta-2,6-dien-1-yl)cyclopentan-1-one|Dithiothreitol|Threitol, 1,4-dithio- (7CI,8CI)|Dithiothreitol, biochemical grade|(2S,3S)-1,4-disulfanylbutane-2,3-diol|LUMEFANTRINE [MI]|1,4-Dimercapto-butane-2,3-diol|2,3-Butanediol, 1,4-dimercapto-, (R*,R*)-|1,4-Dithiothreitol|2,3-Butanediol, 1,4-dimercapto-, (R-(R*,R*))-|D-threo-1,4-Dimercapto-2,3-butanediol|DTV|D-Dtt|D-1,4-Dithiothreitol|DTT (threitol derivative)|threo-2,3-Dihydroxy-1,4-butanedithiol|Cleland's reagent

Storage

Store at 2-8°C,Protected from light,Argon charged

Shipped In

Wet ice

Descripción general

Overview

Reducing agent

Application

Use 1,4-Dithiothreitol primarily to protect free SH-groups from oxidation. Use it routinely in all work with enzymes and proteins during enzyme measurement and the characterization of proteins.

• As one of the reduction and alkylation reactants of the main allergen αs1-casein in milk.

• As a component of sperm membrane removal and reactivation medium.

•Because mercaptans can effectively protect the active sites of biocatalysts and maintain the stability of enzymes.

• As a reducing agent to test the substitution of N-alkaline maleic substitution and the reaction of excess radicals.

Before SDS-PAGE, DTT can effectively reduce protein disulfide bonds in the sample buffer. Both the irritation and toxicity of DTT are reduced by 2-mercaptoethanol. Usually, the required concentration of DTT (100mM) is used. It uses one-seventh of the concentration of 2-cholesterol-based ethanol (5% v/v, 700 mM).

Benefits

1.Rely on the higher stability of DTT in comparison to 2-mercaptoethanol

in aqueous solution

2.Experience more convenience and a less disagreeable odor

3.Benefit from the reduced tendency to oxidize in air.

Specification

Appearance: White to yellowish crystallizate

DTT (with Ellman's reagent): ≥97%

Thin layer chromatography: Chromatographically homogeneous, corresponds to reference

Stability: At +2 to +8°C within specification range for 24 months. Store dry. Protect from light.

Especificaciones

Sinónimos

Threitol, 1,4-dithio-, L- | (+/-)-1,4-Dimercapto-2,3-butanediol | 2-propylpenten-2-oic Acid | dithio threitol | 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel- (9CI) | (+/-)-Dithiothreitol | 2,3-BUTANEDIOL, 1,4-DIMERCAPTO-, L-threo- | DTT, 1,4-Dithiothreitol | 3,7-Dimethyl-6,7-epoxy-1-(4-ethylphenoxy)-2-octene | WR 34678 | (2S,3S)-1,4-DIMER Show more

Especificaciones y pureza

≥97%

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Argon charged

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Pureza

≥97%

Nombres e identificadores

Pubchem Sid

508807088

Sonrisas canónicas

O[C@H](CS)[C@H](O)CS

IUPAC Name

(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

InChIKey

VHJLVAABSRFDPM-QWWZWVQMSA-N

INCHI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

Isómeros SMILES

C([C@H]([C@@H](CS)O)O)S

WGK Alemania

3

RTECS

EK1610000

CAS alternativo

27565-41-9

Peso molecular

154.25

Beilstein

1719760

Reaxy-Rn

9483598

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9483598&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic oxygen compounds
    • Clase Organooxygen compounds
      • Subclass Alcohols and polyols
        • Level5 Polyols
          • Level6 1,2-diols

• Kingdom: Organic compounds
• Superclass: Organic oxygen compounds
• Clase: Organooxygen compounds
• Subclass: Alcohols and polyols
• Intermediate Tree Nodes: Polyols
• Direct Parent: 1,2-diols
• Alternative Parents: Secondary alcohols Alkylthiols Hydrocarbon derivatives
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Secondary alcohol - 1,2-diol - Alkylthiol - Hydrocarbon derivative - Organosulfur compound - Aliphatic acyclic compound
• Descripción: This compound belongs to the class of organic compounds known as 1,2-diols. These are polyols containing an alcohol group at two adjacent positions.
• External Descriptors: 1,4-dithiothreitol

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Objetivos asociados (no humanos)

Trypanosoma brucei brucei (13300 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

pgaB Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (23 Activities)

Discover Target: pgaB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción

Propiedades químicas y físicas

• Solubilidad: Soluble in water, ethanol, chloroform, diethyl ether, acetone, ether and ethyl acetate
• Sensibilidad: Air & Heat and Moisture Sensitive
• Punto de inflamación (°F): 235.4 °F
• Punto de inflamación (°C): 113 °C
• Punto de ebullición (°C): 125～130°C
• Punto de fusión (°C): 42-43°C
• Peso molecular: 154.300 g/mol
• XLogP3: -0.400
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 3
• Exact Mass: 154.012 Da
• Monoisotopic Mass: 154.012 Da
• Topological Polar Surface Area: 42.500 Ų
• Heavy Atom Count: 8
• Formal Charge: 0
• Complexity: 52.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 2
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1