GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant,ExactAb™,Validated,See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human AKT1/AKT2/AKT3 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant AKT1/AKT2/AKT3 Antibody - Primary antibody, specific to AKT1/AKT2/AKT3, IgG
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a
Especies hospedadoras
Rabbit
Especificidad
AKT1/AKT2/AKT3
Inmunógeno
Recombinant protein of human AKT1/2/3 (AA 281-480).
Control positivo
WB: HeLa, Jurkat, NIH/3T3, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - Western Blot All lanes: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 57 kDa Exposure time: 2.7 s
Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
The HeLa cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubated with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) (1/100) at room temperature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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