Goat Anti-Human IgG (HRP) - Secondary antibody

Cat. No.: Ab175835
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 1.0 mg/mL
Application
ELISA, WB
Host species
Goat
Reactive species
Human
Conjugation
HRP
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab175835-100μg
10
$39.90
1mg
Ab175835-1mg
4
$239.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
ELISA: 1:10000-1:100000
WB: 1:10000-1:100000

Specifications

Product Name
Goat Anti-Human IgG (HRP) - Secondary antibody
Synonyms
Goat Anti-Human Antibody (HRP) | HRP-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / HRP antibody | Goat Anti-Human IgG-HRP antibody | Goat Anti-Human IgG-HRP
Specifications & Purity
ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Host species
Goat
Conjugation
HRP
Grade
Azide Free, ExactAb™, High Performance, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Store in the dark.
Images

Goat Anti-Human IgG (HRP) (Ab175835)- ELISA
Goat Anti-Human IgG (HRP) (Ab175835) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (HRP) (Ab175835) was used as the secondary antibody.

Goat Anti-Human IgG (HRP) (Ab175835)- ELISA
Cross-reactivity of Goat Anti-Human IgG (HRP) (Ab175835) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (HRP) (Ab175835) at 1/10000 dilution was used as the primary antibody.

Application
ApplicationDilution info
ELISA

1:10000-1:100000

WB

1:10000-1:100000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot NumberCertificate TypeDateItem
ZJ23F0901190Certificate of AnalysisApr 09, 2026 Ab175835
ZJ23F0901191Certificate of AnalysisApr 09, 2026 Ab175835
ZJ24F0809954Certificate of AnalysisApr 09, 2026 Ab175835
ZJ24F0809955Certificate of AnalysisApr 09, 2026 Ab175835
ZJ24R0500602Certificate of AnalysisMay 25, 2024 Ab175835
Documents & Articles
RNA dot blot protocol
In-cell ELISA protocol
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
Automated Immunohistochemical Staining Protocol for Anti-PD-L1
General Protocol for Western Blotting
BN-PAGE Protocol
Histone Western Blot
Protocol of Western blot for Phosphorylated Proteins
Guidelines of Secondary Antibody for Western Blotting
Multiplex Immunohistochemistry Protocol
Horseradish Peroxidase (HRP) — Structural Features, Enzymatic Properties and Application Overview
Pathogenesis, Virological Characteristics, and Laboratory Detection Strategies for Nipah Virus (NiV)
Tyramide Signal Amplification in In Situ Fluorescence Detection
Protein Carbonyl Detection
Practical Guide to TSA Tyramide Signal Amplification: How to Select Reagents, Optimize the Workflow, and Reduce Background When Detection Signals Are Weak for Low-Abundance Targets
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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