TNF Receptor II/CD120b Armenian Hamster mAb - Primary antibody, specific to TNF Receptor II/CD120b, Armenian Hamster IgG

Cat. No.: Ab131833

GRADE & PURITY Carrier Free, Validated, ExactAb™, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

Blocking, ELISA, Flow Cytometry, Functional Assay, IP, WB

Host species

Armenian hamster

Reactive species

Mouse

Isotype

Armenian Hamster IgG

Clone Number

TR75-54.7

Conjugation

Unconjugated

Overview

Armenian Hamster anti Mouse TNF Receptor II/CD120b Antibody, Monoclonal (TR75-54.7), could be used for IP, WB, Blocking, Flow, ELISA and so on.
Application

IP: Use at an assay dependent concentration.

WB: Use at an assay dependent concentration.

Blocking: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.

ELISA: Use at an assay dependent concentration.
Protein Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Specifications

Product Name

TNF Receptor II/CD120b Armenian Hamster mAb - Primary antibody, specific to TNF Receptor II/CD120b, Armenian Hamster IgG

Synonyms

p75 | TNFBR | Tnfr2 | Tnfr-1 | TNF-alphaR2 | TNFalpha-R2 | CD120b antigen | CD120b | Etanercept | p75 TNF receptor | p75TBPII | p75TNFR | soluble TNFR1B variant 1 | TNF RII | TNFBRp80 TNF-alpha receptor | TNF-R2 | TNFR2TNFR1B | TNF-R75 | TNFR80 | TNFRII |

Specifications & Purity

Carrier Free, ExactAb™, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Host species

Armenian hamster

Specificity

TNF Receptor II/CD120b

Immunogen

Recombinant protein of mouse TNF R2

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, Low Endotoxin, PBS Only, Validated

Isotype

Armenian Hamster IgG

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: TNF Receptor II/CD120b Armenian Hamster mAb (Ab131833)

TNF Receptor II/CD120b Armenian Hamster mAb (Ab131833) - Flow Cytometry
Two-color flow cytometric analysis of TNF Receptor II/CD120b expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Armenian Hamster IgG (Biotin) (Ab191741; Left panel) or TNF Receptor II/CD120b Armenian Hamster mAb (Ab131833; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 versus TNF Receptor II/CD120b (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Blocking:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• Flow Cytometry:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

Associated Targets(Human)

TNFRSF1B Tbio Tumor necrosis factor receptor superfamily member 1B (0 Activities)

Discover Target: TNFRSF1B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action