GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity).
Regulates the recruitment of Langerhans cells to the epidermis, potentially by modula
Host species
Mouse
Specificity
Human Cytokeratin 5
Immunogen
Recombinant protein corresponding to Human Cytokeratin 5
Positive Control
WB: A431, HeLa, HepG2 and SK-OV-3 whole cell lysates IF/ICC: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images
Cytokeratin 5 Mouse mAb (Ab183857) - Western Blot All lanes: Cytokeratin 5 Mouse Antibody (Ab183857) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 58,65 kDa Exposure time: 44.1 s
Cytokeratin 5 Mouse mAb (Ab183857) - ICC/IF IF analysis of Cytokeratin 5 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 5 Mouse mAb (Ab183857) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
1/200
WB
1/1000-1/10000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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