GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Host species
Mouse
Specificity
Phospholipase C beta 1/PLCB1
Immunogen
Recombinant protein of human PLCB1 (AA 31-240)
Positive Control
WB: Rat brain and Mouse brain lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Phospholipase C beta 1/PLCB1 Mouse mAb (Ab121657) - Western Blot All lanes: Phospholipase C beta 1/PLCB1 Mouse mAb (Ab121657) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 139 kDa Observed band size: 100, 150 kDa The study reveals that calpain cleaves PLC-beta 1 at the junction of residues 880 and 881, producing 100 kDa and 45 kDa proteins, with the region between residue 881 and the carboxyl-terminal 10 kDa being essential for G alpha q interaction, while a 140 kDa protein, activated by G alpha q, is not derived from PLC-beta 1.(PMID: 8429045). Exposure time: 56.3 s
Application
Application
Dilution info
WB
1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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