GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Probably involved in nervous system development and function.
Host species
Mouse
Specificity
FGF12
Immunogen
Recombinant protein of human FGF12 (AA 71-240)
Positive Control
WB: Rat brain, Mouse brain lysates. IP: Rat brain lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
FGF12 Mouse mAb (Ab103281) - Western Blot All lanes: FGF12 Mouse mAb (Ab103281) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 22 kDa Exposure time: 157.3 s
FGF12 Mouse mAb (Ab103281) - IP Immunoprecipitation was performed by incubation of 2.5 µg of K94041_8C12 with rat brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. FGF12 Mouse mAb (Ab103281) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Rat brain lysate Lane 2: FGF12 immunoprecipitated from rat brain lysate by FGF12 Mouse mAb (Ab103281) Lane 3: The isotype control antibody instead of FGF12 Mouse mAb (Ab103281) in Rat brain lysate. Result: FGF12 Mouse mAb (Ab103281) can immunoprecipitate FGF12.
Application
Application
Dilution info
WB
1/1000
IP
1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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