Proteinase K, CAS No.39450-01-6

CAS: 39450-01-6 Cat. No.: P109033 EC Number: 254-457-8,3.4.21.64

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%, lyophilized powder,≥30 units/mg protein；expressed in Pichia pastoris

Accession #

P06873

Expression system

Pichia pastoris

Bioactivity

≥30 units/mg protein

Overview

This product is derived from Pichia pastoris expressing the Proteinase K gene from Parengyodontium album (formerly known as Tritirachium album Limber) optimized by site-directed mutagenesis. It exhibits advantages including high specific activity, high yield, and a broader active temperature and pH range.

Advanced protein engineering technology and a 28,000-liter yeast fermentation platform enable efficient unit cost control, providing cost-effective Proteinase K for biopharmaceutical R&D and production. High-efficiency yeast expression and chromatographic purification ensure product purity. The product is endotoxin-free, with a wider range of applications than native Proteinase K.

As one of the highly active proteases available, Proteinase K displays activity over a pH range of 4–12 and a temperature range of 0–75 °C, and retains activity even in the presence of SDS, urea, or EDTA. Proteinase K cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids and is capable of degrading virtually all proteins.

Product Characteristics

Source organism: Pichia pastoris expressing the genetically engineered Proteinase K gene from Parengyodontium album

Molecular weight: 29.3 kDa

pI: 8.9

Applications

Used in gene diagnostic kits, genomic DNA extraction kits, and RNA extraction kits to remove nucleases and other protein contaminants;

Used in the biopharmaceutical industry for the extraction of non-protein biological products such as nucleic acids and polysaccharide drugs, e.g., the preparation of DNA vaccines and heparin;

Used in skin care for the removal of aged keratinocytes, as well as in daily industrial fields including leather tanning, production of hydrolyzed protein seasonings, cosmetics, and feed processing.

Specifications

Product Name

Proteinase K, CAS No.39450-01-6

Synonyms

(4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate | BP178285 | AKOS032949645 | Endopeptidase K | Protease K | Endoproteinase K | Endopeptidase K | peptidase K |

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥95%,lyophilized powder,≥30 units/mg protein；expressed in Pichia pastoris

Biochemical and Physiological Mechanisms

Hydrolyzes keratin at aromatic and hydrophobic residues. Catalytic activity: Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides. Cofactor: Ca2+. Note: Binds 2 calcium ions per subunit.

Bioactivity

≥30 units/mg protein

Accession #

P06873

Predicted molecular weight

29.3 kDa

CAS

39450-01-6

Enzyme Commission Number

EC 3.4.21.64

Molecule Type

Enzyme

Storage and Shipping

Concentration

lyophilized powder,≥30 units/mg protein；expressed in Pichia pastoris

Reconstitution

Reconstitute in 20 mM Tris-HCl, 1 mM CaCl₂, pH 7.4.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

One unit is defined as the enzyme activity that produces 1 µmol of tyrosine per minute from casein at 37°C at pH 7.5.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Lactams
      • Subclass Beta lactams

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Lactams
• Subclass: Beta lactams
• Intermediate Tree Nodes: Not available
• Direct Parent: Carbapenems
• Alternative Parents: Benzyloxycarbonyls Alpha amino acids and derivatives Nitrobenzenes Pyrroline carboxylic acids Phenoxy compounds Phenol ethers Nitroaromatic compounds Monoalkyl phosphates Azepines Alkyl aryl ethers Vinylogous esters Tertiary carboxylic acid amides Enoate esters Secondary alcohols Azetidines Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Monocarboxylic acids and derivatives Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Carbapenem - Benzyloxycarbonyl - Alpha-amino acid or derivatives - Nitrobenzene - Phenoxy compound - Nitroaromatic compound - Pyrroline carboxylic acid or derivatives - Pyrroline carboxylic acid - Phenol ether - Monoalkyl phosphate - Azepine - Alkyl aryl ether - Benzenoid - Alkyl phosphate - Phosphoric acid ester - Organic phosphoric acid derivative - Monocyclic benzene moiety - Vinylogous ester - Alpha,beta-unsaturated carboxylic ester - Enoate ester - Tertiary carboxylic acid amide - Pyrroline - Organic nitro compound - Secondary alcohol - C-nitro compound - Carboxylic acid ester - Carboxamide group - Azetidine - Azacycle - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Monocarboxylic acid or derivatives - Ether - Carboxylic acid derivative - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Carbonyl group - Alcohol - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as carbapenems. These are beta-lactam derivatives in which the beta-lactam ring shares the nitrogen atom with a pyrrole-2-carboxylic acid.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y