Recombinant CD45RO Antibody (PE) - Primary antibody, specific to Human CD45RO

Cat. No.: Ab213950

GRADE & PURITY Validated, ExactAb™, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

P08F59

Conjugation

PE

Overview

Recombinant CD45RO Antibody, clone P08F59, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45RO Antibody (PE) - Primary antibody, specific to Human CD45RO

Synonyms

B220 | CD45 antigen | CD45R | CD45RO | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor type, c polypeptide | B220 a

Specifications & Purity

ExactAb™, Validated, 5 μL/test

Biochemical and Physiological Mechanisms

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the secon

Host species

Mouse

Specificity

Human CD45RO

Immunogen

IL-2 dependent T cell line, CA1

Positive Control

Flow Cytometry: Human peripheral blood lymphocytes.

Conjugation

PE

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

5 μL/test

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD45RO Antibody (PE) (Ab213950)

Recombinant CD45RO Antibody (PE) (Ab213950) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45RO Antibody (PE) (Ab213950) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE) (Ab180308).

Flow Cytometry: Recombinant CD45RO Antibody (PE) (Ab213950)

Recombinant CD45RO Antibody (PE) (Ab213950) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45RO Antibody (PE) (Ab213950) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)