ExactAb™, Validated, Recombinant, High performance, 2mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human ERK1 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC and so on.
Recombinant ERK1 Antibody (Ab102223) - Western Blot
All lanes: Recombinant ERK1 Antibody (Ab102223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 27.0 s
Recombinant ERK1 Antibody (Ab102223) - IF/ICC
Recombinant ERK1 Antibody (Ab102223) staining ERK1 in HeLa cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant ERK1 Antibody (Ab102223) (1/50) at 4°C. An AF488-conjugatedGoat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF488-conjugatedGoat Anti-Rabbit IgG (1/500).
Recombinant ERK1 Antibody (Ab102223) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ERK1 Antibody (Ab102223) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ERK1 Antibody (Ab102223) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant ERK1 Antibody (Ab102223) - IP
ERK1 was immunoprecipitated from 0.4 mg of A375 whole cell lysate with Recombinant ERK1 Antibody (Ab102223) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ERK1 Antibody (Ab102223) IP in A375 whole cell lysate
Lane 2: PBS instead of Recombinant ERK1 Antibody (Ab102223) in A375 whole cell lysate
Lane 3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.
Recombinant ERK1 Antibody (Ab102223) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded
sections) analysis Cervix cancer tissue labelling ERK1 with Recombinant ERK1 Antibody (Ab102223) at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
| Aplicación | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| IF/ICC | 1/50 - 1/200 |
| IP |
1/50
|
| Flow Cytometry |
1/200 - 1/1000
|
| WB |
1/1000 - 1/10000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ24F0404288 | Certificate of Analysis | Feb 10, 2026 | Ab102223 |
| ZJ23F0901102 | Certificate of Analysis | Sep 18, 2023 | Ab102223 |
| ZJ23F0901103 | Certificate of Analysis | Sep 18, 2023 | Ab102223 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
