CD3 Mouse mAb (Biotin) - Primary antibody, specific to CD3E, Mouse IgG2a

Cat. No.: Ab179703

GRADE & PURITY Validated, ExactAb™, Azide Free, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

HIT3a

Conjugation

Biotin

Descripción general

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD3 Mouse mAb (Biotin) - Primary antibody, specific to CD3E, Mouse IgG2a

Sinónimos

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Especificaciones y pureza

ExactAb™, Validated, Azide Free, 0.5 mg/mL

Especies hospedadoras

Mouse

Especificidad

CD3E

Inmunógeno

Human thymocytes

Control positivo

Flow: Jurkat

Conjugación

Biotin

Grado

Azide Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD3 Mouse mAb (Biotin) (Ab179703)

CD3 Mouse mAb (Biotin) (Ab179703) - Flow Cytometry
Flow cytometry analysis of CD3 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (Biotin) (Ab179703) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción