Recombinant CD3 Antibody - Primary antibody, specific to Human CD3ε

Cat. No.: Ab219596

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

P93B35

Conjugation

Unconjugated

Descripción general

Recombinant CD3ε Antibody, clone P93B35, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

Recombinant CD3 Antibody - Primary antibody, specific to Human CD3ε

Especificaciones y pureza

Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Mecanismos bioquímicos y fisiológicos

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b

Especies hospedadoras

Mouse

Especificidad

Human CD3ε

Inmunógeno

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C long time. Upon delivery aliquot. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Recombinant CD3 Antibody (Ab219596)

Recombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab219596) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with AF 488-conjugated affinipure goat anti-Mouse IgG.

Flow Cytometry: Recombinant CD3 Antibody (Ab219596)

ecombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab219596) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with AF 488-conjugated affinipure goat anti-Mouse IgG. Blue - Isotype control, compared with Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD3 Antibody (Ab219596)

Recombinant CD3 Antibody (Ab219596) - SEC
The purity of Recombinant CD3 Antibody (Ab219596) is more than 95% verified by HPLC.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.5 µg per million cells in 100 µl volume;

Objetivos asociados (humanos)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)