CD3 Mouse mAb (PE-Cy5) - Primary antibody, specific to CD3

Cat. No.: Ab189665

GRADE & PURITY Validated, ExactAb™, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

PE-Cy5

Descripción general

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow Cytometry

Specifications

Product Name

CD3 Mouse mAb (PE-Cy5) - Primary antibody, specific to CD3

Sinónimos

CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a

Especificaciones y pureza

ExactAb™, Validated, 5 μL/test

Mecanismos bioquímicos y fisiológicos

The CD3 complex mediates signal transduction.

Especies hospedadoras

Mouse

Especificidad

CD3

Inmunógeno

Human CD3ε

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

PE-Cy5

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

670nm

Ex/Em(nm)

Ex:565nm;Em:670nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

5 μL/test

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD3 Mouse mAb (PE-Cy5) (Ab189665)

CD3 Mouse mAb (PE-Cy5) (Ab189665) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (PE-Cy5) (Ab189665) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5) (Ab190078).

Flow Cytometry: CD3 Mouse mAb (PE-Cy5) (Ab189665)

CD3 Mouse mAb (PE-Cy5) (Ab189665) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (PE-Cy5) (Ab189665) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Objetivos asociados (humanos)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)