Cyclophilin A Mouse mAb - Primary antibody, specific to Human Cyclophilin A

Cat. No.: Ab183902

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

EF01/4D12

Conjugation

Unconjugated

Overview

Mouse anti Human Cyclophilin A Antibody, clone EF01/4D12, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name

Cyclophilin A Mouse mAb - Primary antibody, specific to Human Cyclophilin A

Synonyms

Cyclophilin A antibody | Cyclophilin antibody | CyclophilinA antibody | Cyclosporin A binding protein antibody | Cyclosporin A-binding protein antibody | CYPA antibody | CYPH antibody | Epididymis secretory sperm binding protein Li 69p antibody | HEL S 69

Specifications & Purity

Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Biochemical and Physiological Mechanisms

Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates i

Host species

Mouse

Specificity

Human Cyclophilin A

Immunogen

Recombinant protein corresponding to Human Cyclophilin A

Positive Control

WB: HeLa, K562, HepG2, A549, Jurkat, A431and NIH/3T3 whole cell lysates; Mouse brain and Rat brain tissue Flow Cytometry: HeLa cells

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Light Chain Type

kappa

SDS-PAGE

150.0 KDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: Cyclophilin A Mouse mAb (Ab183902)

Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 60.0 s

Western Blot: Cyclophilin A Mouse mAb (Ab183902)

Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 90.0 s

Western Blot: Cyclophilin A Mouse mAb (Ab183902)

Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 90.0 s

Flow Cytometry: Cyclophilin A Mouse mAb (Ab183902)

Cyclophilin A Mouse mAb (Ab183902) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Cyclophilin A Mouse mAb (Ab183902) (Red) at 5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).

Application

• Application: Dilution info
• WB:

  1/1000 - 1/20000 ;

• Flow Cytometry:

  1/200 - 1/3000

Associated Targets(Human)

PPIA Tclin Peptidyl-prolyl cis-trans isomerase A (0 Activities)

Discover Target: PPIA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)