ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti Human GAPDH Antibody, Monoclonal (B08/4A11), could be used for WB; IF/ICC and Flow Cytometry.
GAPDH Mouse mAb (Biotin) (Ab219252) - Western Blot
All lanes: GAPDH Mouse mAb (Biotin) (Ab219252) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 127.5 s
GAPDH Mouse mAb (Biotin) (Ab219252) - Flow Cytometry
Flow cytometry analysis of GAPDH expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (Biotin) (Ab219252) (Red). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
GAPDH Mouse mAb (Biotin) (Ab219252) – IF/ICC
IF analysis of GAPDH (green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Biotin) (Ab219252) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/100-1/1000
|
| Flow Cytometry | 1/1000 - 1/10000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ25F0521670 | Certificate of Analysis | Mar 16, 2026 | Ab219252 |
| ZJ25F0521671 | Certificate of Analysis | Mar 16, 2026 | Ab219252 |
| ZJ25F0521672 | Certificate of Analysis | Mar 16, 2026 | Ab219252 |
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